Flow Cytometry for Biotechnology

Framsida
Larry A. Sklar
Oxford University Press, 2 sep. 2005 - 400 sidor
0 Recensioner
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.
 

Så tycker andra - Skriv en recension

Vi kunde inte hitta några recensioner.

Innehåll

1 The Future of Flow Cytometry in Biotechnology
3
Tools for Flow Cytometry
13
Biological Applications
179

Andra upplagor - Visa alla

Vanliga ord och fraser

Populära avsnitt

Sidan 371 - R (1989) Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell 59...
Sidan 373 - Springer, TA (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301-314.
Sidan 370 - Vascular cell adhesion molecule-1 (VCAM-1) gene transcription and expression are regulated through an antioxidant-sensitive mechanism in human vascular endothelial cells. J Clin Invest 92:186674, 1993.
Sidan 370 - Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes and other leukocytes. J.
Sidan 373 - Staunton. DE, Dustin. ML. Springer, TA (1989) Functional cloning of ICAM-2. a cell adhesion ligand for LFA-1 homologous to ICAM-1. Nature 339.

Bibliografisk information