GB 4789.35-2016 Translated English of Chinese Standard. GB 4789.35-2016: National food safety standard - Microbiological examination of food - Examination of lactic acid bacteriahttps://www.chinesestandard.net, 15 sep. 2017 - 15 sidor This standard specifies the testing method of the lactic acid bacteria in the food containing lactic acid bacteria. This standard is applicable to the testing of lactic acid bacteria in foods containing active lactic acid bacteria. |
Innehåll
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Scope | 4 |
Testing procedures | 5 |
Operating procedures | 6 |
Results and reports | 10 |
Appendix A Medium and reagents | 12 |
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1:10 sample homogenate ABSORB 1 mL Aescinate agar culture medium anaerobic environment autoclaved at 121 average number Beef powder Bifidobacterium number individually calculated by multiplying Carbohydrate containing 225 mL continuous appropriate dilutions cooled to 48 counting Bifidobacterium number Crystal violet Cysteine Hydrochloride modified dilution factor duration from sample environment for 72 fermentation tube finished within 15 Glucose Gram stain HEAT to dissolve lactic acid bacteria Lactose Li-Mupirocin and Cysteine Mannitol Methodology ADD micro pipette mix it uniformly mL distilled water mL of physiological mL of sample mL sterile pipette modified MRS medium multiplying the average neutral red Normal saline number of colonies number of lactic number of Streptococcus numeration culture condition Operate in accordance physiological saline plate pouring plate to mix produce 1:10 sample requires counting Bifidobacterium result description ROTATE the plate sample dilution selection and result Sorbitol sterile homogeneous sterile plate strains are positive transferring the diluent Yeast powder