GB 4789.42-2016 Translated English of Chinese Standard. GB4789.42-2016: National Food Safety Standard - Food microbiological examination - Examination of norovirushttps://www.chinesestandard.net, 4 nov. 2017 - 23 sidor This standard specifies real-time fluorescent RT-PCR detection method of norovirus in food. This standard applies to the norovirus nucleic acid detection in hard-surfaced foods such as shellfish, raw vegetables, carrots, melons, nuts and so on AND such soft foods as the strawberries, tomatoes, grapes and so on. |
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ADD 5 µL added amplification control amplification control RNA blank control buffer solution centrifuge tube contained food sample control is negative control virus contained control virus primer control virus RNA cotton swab Ct value diluted food sample diluted process control ethanol externally added amplification extraction efficiency extraction process control fluorescent RT-PCR primers fluorescent RT-PCR reaction fold diluted food fold dilution process food sample RNA g RNase-free GenBank access number genetically modified organism GII type primer hard-surfaced food Ingredients inhibition index judged as norovirus MIX it uniformly mmol/L negative control norovirus real-time fluorescent oscillation pectinase plasmid Polyethylene glycol Preparation method DISSOLVE primers and probes process control virus r/min raw vegetables reaction hole real-time fluorescent RT-PCR Restriction enzymes RNA purification reagent RNase RNase-free ultrapure water room temperature RT-PCR reaction system shellfish Sodium chloride soft fruit speed subsequent RNA extraction supernatant type primer probe virus contained food virus extraction process virus primer probe