GB 4789.43-2016 Translated English of Chinese Standard. GB 4789.43-2016: National Food Safety Standard - Food microbiological examination - Microbial source enzyme preparation antibacterial activity determinationhttps://www.chinesestandard.net, 11 aug. 2017 - 10 sidor This Standard specifies the method for the determination of antibacterial activity of microbial source enzyme preparation. This Standard is applicable to the determination of antibacterial activity of enzyme preparation produced by microorganism. |
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1°C constant temperature 10-4 dilution solution 6.3 Preparation 9 mL Accurately weigh pipet Antimicrobial activity report bacillus cereus bacteria suspension solution bacteria suspension stock circulans ATCC 4516 containing 100 µL containing test enzyme control paper containing cooled to 46°C Cultivate at 36°C Cultivate at 36°C±1°C Determination of concentration determination Report Figure Distilled water Enzyme preparation paper enzyme preparation sample enzyme preparation solution escherichia coli inoculation on TSA inspection plate prepared mL of TSB negative control paper paper containing ciprofloxacin paper containing test petri dish pieces of paper positive control preparation into 9 Preparation method Pipet preparation paper Accurately prepared in 6.3 prepared in 6.4 prepared to 10-5 pyogenes ATCC 12344 Respectively pick respectively prepared Sodium chloride solution evenly paved solution prepared staphylococcus aureus sterile paper sterile plate sterile saline stock solution Respectively streptococcus pyogenes ATCC suspension solution pavement suspension stock solution test bacteria suspension test enzyme preparation TSA medium TSA plate