GB 5009.128-2016: Translated English of Chinese Standard. GB5009.128-2016: National food safety standard - Determination of cholesterol in foodsThis Standard specifies the determination methods of cholesterol in foods. This Standard applies to the determination of cholesterol in foods. Method One Gas chromatography is applicable to the determination of cholesterol in meat and meat products, eggs and egg products, milk and dairy products and other animal foods and vegetable oils and fats. Method Two High performance liquid chromatography is applicable to the determination of cholesterol in meat and meat products, eggs and egg products, milk and dairy products and other animal foods. Method Three Colorimetric method is applicable to the determination of cholesterol in meat and meat products, eggs and egg products and other animal foods. |
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3 | |
Scope | 4 |
Instruments and equipment | 5 |
Expression of analysis result | 7 |
Precision | 8 |
Instruments and equipment | 9 |
Expression of analysis result | 11 |
Precision | 12 |
Instruments and equipment | 14 |
Expression of analysis result | 15 |
Annex A Cholesterol standard chromatogram | 16 |
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4°C sealing absolute ethanol alum color solution anhydrous ether mixture anhydrous sodium sulfate arithmetic mean boiling range 30°C bottle wall cholesterol content cholesterol in foods Cholesterol standard C27H46O cholesterol standard product Cholesterol standard series Cholesterol standard stock Chromatographic column content of cholesterol Determination of cholesterol dissolve and set ethanol to dissolve ether and anhydrous ether mixture 1+1 Expression of analysis external standard method extract flask in 2~3 g of cholesterol gas chromatography glacial acetic acid High performance liquid hydrochloric acid independent determinations obtained Meat and meat meat products milliliters mL mL of absolute mL of water nearest to 0.1 obtained under repeatability performance liquid chromatography petroleum ether pipette potassium hydroxide solution rinse the round-bottomed round-bottomed flask sample by absolute sample mass sample solution separatory funnel set volume sodium chloride sodium hydroxide solution 1.0 mg/mL standard curve standard product nearest standard stock solution stock solution 1.0 temperature volume to 100