GB 5009.285-2022 Translated English of Chinese Standard (GB5009.285-2022): National food safety standard - Determination of Vitamin B12 in foods
https://www.chinesestandard.net, 21 aug. 2022 - 27 sidor
This Standard specifies the determination method of vitamin B12 in foods. Method I of liquid chromatography applies to the determination of vitamin B12 in foods for infants and young children, milk and milk products, meat and meat products.
absorbance according accurate to 0.01 acid activity adjust Appendix B12 isotope internal blank brown reagent bottle calculate centrifuge chromatographic components concentration of vitamin cyanide or sodium cyanocobalamin dark detection determination of vitamin discard dissolve Draw ethanol solution exceed Figure filter fix the volume flow foods for infants glucose immunoaffinity column infants and young isotope internal standard Lactobacillus light transmittance limit Liquid chromatography mark Materials measure medium method milk milliliter mL of vitamin mL of water mL volumetric flask mol/L ng/mL Note obtained operate passing phase pipette potassium cyanide Preparation r/min range relative respectively sample extract sample solution sampling amount shake sodium cyanide sodium hydroxide solution to fix specified standard curve standard solution standard substance standard working solution Sterilize Table test tube to-be-tested solution transfer valid Vitamin B12 isotope vitamin B12 standard water bath water to dilute Weigh young children